Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics

Figure 3

Verification of biomarker candidates using SQ-MRM analysis. To verify the selected biomarker candidates, we measured the relative concentrations of the 39 candidates in the samples from the No DR, Mi NPDR, and Mo NPDR groups. In the SQ-MRM analysis, we confirmed a differential measurement of these proteins among the No DR (), Mi NPDR (), and Mo NPDR () groups. ROC with AUC values were generated based on the SQ-MRM analysis. In the SQ-MRM analysis, we “spiked” the samples with 50 fmol betaGAL (GDFQFNISR, 542.3/636.3 m/z).