Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics

Figure 6

Representative calibration curves. To generate calibration curves for the 15 proteins, heavy peptides were serially diluted (12 concentrations, 0.1–200 fmol) with the light peptide as an internal standard (digested pooled plasma protein, 1 μg) that was added into each serially diluted sample. A representative calibration curve for APOA4_LAPLAEDVR (y2⁺⁺) is shown. Insets show the linear ranges of the curves using ≥ 0.99 to determine the LLOQ.