Antidiabetic Effect of Young and Old Ethanolic Leaf Extracts of Vernonia amygdalina: A Comparative Study

Figure 2

Photomicrograph of liver sections. (a) Normal control: normal polygonal shape of hepatocytes, devoid of cellular injuries. (b) Negative control: it shows fatty change (cytoplasmic halo), slightly edematous tissue section, activated Kupffer cells, and basophilic and pyknotic nuclei of some hepatocytes surrounding the central vein (CV). (c) Positive control: normal hepatocytes with increased eosinophilia, accompanied with mild leucocytosis. (d) 10 mg/kg YL: normal hepatocytes with focal areas of necrosis. (e) 10 mg/kg OL: reduced fatty change as compared to the negative control, with mild oedema. (f) 30 mg/kg YL: section depicts normal cells of the liver, with reduced eosinophilia of cytoplasm of hepatocytes. (g) 30 mg/kg OL extract: focal areas of necrosis with reduced fatty change. (h) 300 mg/kg YL: focal areas of necrosis with mark sinusoidal leucocytosis. (i) 300 mg/kg OL extract: normal hepatocytes with very mild leucocytosis (H&E stain 100x). Bar = 20 μm.

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