Antidiabetic Effect of Young and Old Ethanolic Leaf Extracts of Vernonia amygdalina: A Comparative Study

Figure 4

Photomicrograph of histological sections of pancreas. (a) Normal control: section shows normal histology of pancreas with high cellularity of the islet of Langerhans. (b) Negative control: islets show highly reduced cellularity of the endocrine islets with inter- and intracytoplasmic vacuolations. (c) Positive control: it has normal arrangement of islet cells and the cellularity is likened to that of the normal control. (d) 10 mg/kg YL: reduction of cells in the middle portion of the islets of Langerhans. Sign of little regeneration. (e) 10 mg/kg OL: it also shows atrophy of islet with little regeneration of resident cells. (f) 30 mg/kg YL: islet has increased number of cells as compared to the normal control but being less compact. (g) 30 mg/kg OL: it also shows sign of regeneration but cellularity is less pronounced as compared to the 30 mg/kg YL. (h) 300 mg/kg YL: it shows sign of total regeneration of cells of the islet. (i) 300 mg/kg OL: it displays sign of total regeneration of the islets of Langerhans (H&E stain 100x). Bar = 20 μm.

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