Metformin Treatment Prevents Sedentariness Related Damages in Mice

Figure 3

METF action on C2C12 proliferation phase. (a) METF did not modify C2C12 growth rate and did not induce cytotoxic effects. (b) METF increased MyoD protein content with respect to the control. (c) Phase contrast images at the end of proliferation phase. (d) Immunofluorescence analysis of early MRFs MyoD and Myf5 (24 h): METF enhanced myocytes committed. (e) Phalloidin assay at 48 h of proliferation. Representative immunoblots of analyzed proteins are shown. Scale bar 200 μm. Significance: .

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