Central Administration of Galanin Receptor 1 Agonist Boosted Insulin Sensitivity in Adipose Cells of Diabetic Rats

Figure 5

The i.c.v. administration of M617 enhanced GLUT4 contents and trafficking to plasma membranes of fat cells (). (a) The GLUT4 immunoreactivity in total cell membranes and plasma membranes was higher in diabetic M617 (D-M617) and nondiabetic M617 (N-M617) groups as compared with diabetic controls (DC) and nondiabetic controls (NC), respectively. The GLUT4 immunoreactivity in D-M617 and DC was lower as compared with N-M617 and NC, respectively, in total cell membranes and plasma membranes of adipose cells. (b) The central treatment with M617 enhanced the ratios of GLUT4 levels in plasma membranes to total cell membranes in D-M617 and N-M617 compared with each control, respectively. As compared with the diabetic controls, the ratio in N-M617 was increased, but in DC it was reduced. The data shown are the means ± SEM. , versus NC; , versus N-M617; , versus DC.

(a)

(b)