The Relation between Periodontopathogenic Bacterial Levels and Resistin in the Saliva of Obese Type 2 Diabetic Patients

Table 1

List of the primers used in real-time PCR experiments with the annealing temperatures for each assay.

Bacterial species (target gene)	Primer sequence (5–3)	Annealing temperature (°C)	Reference
Actinobacillus actinomycetemcomitans (iktA)	CAG CAT CTG CGA TCC CTG TA	58	[13]
	TCA GCC CTT TGT CTT TCC TAG GT
Porphyromonas gingivalis (16S rRNA)	ACC TTA CCC GGG ATT GAA ATG	58	[14]
	CAA CCA TGC AGC ACC TAC ATA GAA
Treponema denticola (16S rRNA)	AGA GCA AGC TCT CCC TTA CCG T	58	[15]
	TAA GGG CGG CTT GAA ATA ATG A
Tannerella forsythia (16S rRNA)	GGG TGA GTA ACG CGT ATG TAA CCT	55.5	[16]
	ACC CAT CCG CAA CCA ATA AA
Fusobacterium spp. (16S rRNA)	CGC AGA AGG TGA AAG TCC TGT AT	58	[17]
	TGG TCC TCA CTG ATT CAC ACA GA
Universal primers (16S rRNA)	TCC TAC GGG AGG CAG CAG T	60	[18]
	GGA CTA CCA GGG TAT CTA ATC CTG TT