The Imbalance of B-Lymphocyte Subsets in Subjects with Different Glucose Tolerance: Relationship with Metabolic Parameter and Disease Status

Figure 1

Representative flow cytometry analysis of peripheral B-cell subsets in subjects with NGT, IGR, and T2D. (a) Dots represent CD19⁺ B-cell frequency in total lymphocytes. Dead cells were excluded from the analysis based on their forward- and side-light scatter properties and propidium iodide staining. Doublets were excluded by FSC-A/FSC-H. (b) Representative dot plot showed the gating strategy for B-2 and B-1(B-1a, B-1b) cells in the CD19⁺ gate. (c) Representative dot plot showed the gating strategy for B10 cells gated on CD19⁺ B cells. IGR, impaired glucose regulation subjects; NGT, normal glucose tolerance subjects; T2D, type 2 diabetic subjects.

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