Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro

Figure 1

Overexpressed proinsulin identified in EML cells. (a), (b) GFP-positive cells were detected via FACS in EML cells transduced by lentivirus particles carrying empty vector (empty) or proinsulin gene (Pro-INS), respectively. (c) Overexpressed proinsulin mRNA was detected using real-time PCR in EML cells transduced by LV-V carrying the proinsulin gene. (d) Proinsulin protein was detected via Western blotting in EML cells transduced by LV-V carrying the proinsulin gene.

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