Aldose Reductase Inhibitor Protects against Hyperglycemic Stress by Activating Nrf2-Dependent Antioxidant Proteins

Figure 2

AR inhibition augments HG- induced Nrf2 activation in Thp1 cells. Thp1 cells were treated with fidarestat (10 μM) for indicated times. Subsequently, the cells were also pretreated with fidarestat for overnight followed by incubation with HG for 30, 60, 120, and 240 minutes. Equal amounts of nuclear and cytosolic proteins were subjected to Western blot analysis for the expression of Nrf2 and Keap1, respectively. Histone H3 and GAPDH served as loading controls for nuclear and cytosolic protein extract, respectively. A representative blot from three independent analyses is shown (a). Nrf2 transcription factor assay using the nuclear protein of treated Thp1 cells was carried out using an ELISA kit (b). Data represent mean ± SD (). when compared with control, and # when compared with the HG-treated group.

(a)

(b)