Diosgenin, a Novel Aldose Reductase Inhibitor, Attenuates the Galactosemic Cataract in Rats

Figure 1

Optimization of ARIs screening method and screening results of ARIs. (a) The scanning plots of different combinations of all components from 200 nm to 999 nm. (b) Inhibition of epalrestat (10⁻¹¹–10⁻⁶ mol/L) on normal rat lens AR with good dose-dependent manner. (c) Dose-response curve of epalrestat on rat lens AR based on the result of 10 minutes, IC₅₀ = 1.32 × 10⁻⁸ mol/L. (d) ARIs screening results of 224 candidates, diosgenin was the strongest ARI. (e) Chemical structure of diosgenin, MW = 414.61. (f) Dose-response curve of diosgenin on rat lens AR, IC₅₀ = 4.59 × 10⁻⁶ mol/L.

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