The LDL Receptor-Related Protein 1: At the Crossroads of Lipoprotein Metabolism and Insulin Signaling

Table 1

t_1/2 values calculated from rate constants for LRP1 recycling in cells.

Ligand	Cell	Condition	t1/2 endocytosis (min)	Fold	t1/2 exocytosis (min)	Fold	^aPM	Fold	Ref
AF647-α2M	3T3-L1 cells	Basal	1.69	1.0	6.93	1.0	0.22	1.0	[54]
AF647-α2M	3T3-L1 cells	Insulin	1.69	1.0	6.30	0.9	0.24	1.1	[54]
AF647-α2M	3T3-L1 differentiated	Basal	2.48	1.5	33.00	4.8	0.07	0.3	[54]
AF647-α2M	3T3-L1 differentiated	Insulin	2.04	1.2	16.12	2.3	0.11	0.5	[54]
^125I-8G1	WI-38 fibroblasts	Basal	4.81	2.8	ND	ND	ND	ND	[71]
^125I-α2M	Human SMCs	Basal	1.08	0.6	ND	ND	ND	ND	^b
^125I-α2M	CHO LRP1 null transfected; with chicken LRP1	Basal	2.68	1.6	ND	ND	ND	ND	[72]

^aFraction of total expressed in plasma membrane [54]. ^bM. Migliorini, S. C. Muratoglu, D. T. Au, and D. K. Strickland, unpublished data. ND: not determined.