Reduced Histone H3 Lysine 9 Methylation Contributes to the Pathogenesis of Latent Autoimmune Diabetes in Adults via Regulation of SUV39H2 and KDM4C

Figure 1

The study scheme. Twenty-six LADA patients and 26 healthy control subjects were enrolled in this study. Peripheral blood mononuclear cells (PBMC) were isolated with Ficoll from peripheral venous blood which was obtained from each participant. CD4⁺ T lymphocytes were isolated by flow cytometry. Then, Global H3 lysine 4 and H3 lysine 9 methylation was detected in CD4⁺ T lymphocytes from 26 subjects. The mRNA level of histone methyltransferases gene (SET1, SUV39H1, SUV39H2, and G9a) and histone demethylases gene (LSD1, KDM3A, KDM4A, KDM4B, KDM4C, KDM5A, KDM5B, KDM5C, and KDM5D) was detected by real-time PCR.