Meprin Metalloprotease Deficiency Associated with Higher Mortality Rates and More Severe Diabetic Kidney Injury in Mice with STZ-Induced Type 1 Diabetes

Figure 7

Localization of meprin B in the juxtamedullary region of the kidney (a), acid Schiff-stained kidney sections (b), and representative immunoblot for villin in BBM-enriched kidney protein fractions from wild-type and meprin αβKO mice at 18 weeks post-STZ (c). There was no noticeable redistribution of meprins from the BBMs to other cell compartments. Significant tubular dilation was evident in the kidneys from diabetic mice when compared to that from nondiabetic controls. There was more tubular dilation in STZ-treated mice when compared to that in nondiabetic vehicle-treated controls. The levels of villin were significantly lower in diabetic WT mice when compared to those in nondiabetic controls (). This decrease was not observed in meprin αβKO mice. .

(a)

(b)

(c)