Research Article
Metformin Improves Epithelial-to-Mesenchymal Transition Induced by TGF-β1 in Renal Tubular Epithelial NRK-52E Cells via Inhibiting Egr-1
Table 1
Sequences of primers used for real-time quantitative PCR.
| | Genes | Forward | Reverse |
| | Egr-1 | 5-CCAGTGCCCACCTCTTACTC-3 | 5-TGCAGACTGGAAGGTGCTG-3 | | Fibronectin | 5-CATGGCTTTAGGCGAACCA-3 | 5-CATCTACATTCGGCAGGTATGG-3 | | E-cadherin | 5-TGATGATGCCCCCAACACTC-3 | 5-CCAAGCCCTTGGCTGTTTTC-3 | | α-SMA | 5-GACCCTGAAGTATCCGATAGAACA-3 | 5-CACGCGAAGCTCGTTATAGAAG-3 | | β-Actin | 5-GCGAGTACAACCTTCTTGCAG-3 | 5-GCCTTGCACATGCCGGA-3 |
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