MicroRNA-22 Promotes Renal Tubulointerstitial Fibrosis by Targeting PTEN and Suppressing Autophagy in Diabetic Nephropathy

Figure 6

PTEN partially mediated the biological effect of miR-22 on autophagy and the synthesis of Col IV. (a) After transfected with PTEN overexpression plasmid or empty plasmid for 24 h, NRK-52E cells were cultured with NG or HG for another 48 h. The expression of PTEN, p-Akt, t-Akt, p-mTOR, t-mTOR, LC3-I, LC3-II, p62, and Col IV was detected by Western blot. p-Akt: phosphorylated Akt; t-Akt: total Akt; p-mTOR: phosphorylated mTOR; t-mTOR: total mTOR. ImageJ densitometric analysis of the protein levels; data represent mean ± SEM, , , . (b) After cotransfected with PTEN overexpression plasmid or empty plasmid and miR-22 mimics or NC mimics for 24 h, NRK-52E cells were treated with NG or HG for another 48 h. The expression of LC3-I, LC3-II, p62, and Col IV was detected by Western blot. ImageJ densitometric analysis of the protein levels; data represent mean ± SEM, , , , .

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