The Iron-Klotho-VDR Axis Is a Major Determinant of Proximal Convoluted Tubule Injury in Haptoglobin 2-2 Genotype Diabetic Nephropathy Patients and Mice

Figure 7

1-α-Hydroxylase expression in diabetic and nondiabetic (nonDM) mice. (a) Slides from mice renal biopsies were subjected to IHC of 1-α-hydroxylase. (b) There was a significant increase in the percentage of the stained area of 1-α-hydroxylase in the tubules of Hp 2-2 DM renal sections compared with Hp 1-1 DM sections () but insignificant compared with nonDM Hp 2-2 genotype. (c) Western blot analysis of 1-α-hydroxylase with the mice renal lysate. Proteins were detected by immunoblotting with antibodies against 1-α-hydroxylase and GAPDH. The values represent the mean ± SEM for 3 independent experiments subjected to 2-tailed, 2-sampled unequal variance Student’s t-test, with compared with Hp 1-1DM. AU: arbitrary units.

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