Food Intake and Core Body Temperature of Pups and Adults in a db Mouse Line Deficient in the Long Form of the Leptin Receptor without Misty Mutation
Table 2
Organ weight and biochemical parameters including hormones at 2 and 8 weeks.
2 weeks
8 weeks
values (two-way ANOVA)
Male
WT ()
db/db ()
WT ()
db/db ()
Age
Genotype
Interaction
Body weight
g
6.15 ± 0.18a
6.41 ± 0.21a
24.91 ± 0.30b
41.82 ± 0.75c
(<0.01)
(<0.01)
<0.01
Milk intake#
g/h
0.146 ± 0.015
0.153 ± 0.024NS
Food intake
g/day
3.42 ± 0.09
7.60 ± 0.33
Organs
Brain
mg
355.0 ± 5.6ab
335.5 ± 5.9a
421.7 ± 3.6c
363.3 ± 5.1b
(<0.01)
(<0.01)
<0.01
Heart
mg
34.8 ± 2.0
30.0 ± 1.1
120.9 ± 1.5
117.9 ± 2.8
<0.01
0.055
0.660
Liver
g
0.19 ± 0.01a
0.19 ± 0.01a
1.36 ± 0.03b
2.79 ± 0.11c
(<0.01)
(<0.01)
<0.01
WAT
g
0.013 ± 0.002a
0.026 ± 0.003a
0.250 ± 0.013b
1.781 ± 0.073c
(<0.01)
(<0.01)
<0.01
BAT
mg
61.0 ± 6.5
76.5 ± 6.3
103.2 ± 8.0
198.9 ± 49.4
<0.01
0.037
0.126
Plasma
Glucose
g/l
1.12 ± 0.05a
1.01 ± 0.04a
2.29 ± 0.19a
6.14 ± 0.89b
(<0.01)
(<0.01)
<0.01
TG
g/l
0.68 ± 0.10
0.79 ± 0.12
1.04 ± 0.13
1.20 ± 0.17
<0.01
0.284
0.844
Insulin
ng/ml
0.40 ± 0.03a
0.59 ± 0.08a
0.99 ± 0.09a
9.61 ± 3.53b
(<0.01)
(<0.019)
0.024
Leptin
ng/ml
4.0 ± 1.1a
46.6 ± 5.4b
1.1 ± 0.3a
94.6 ± 7.2c
(<0.01)
(<0.01)
<0.01
Corticosterone
ng/ml
43.6 ± 7.1a
56.3 ± 8.7a
148.0 ± 33.2a
292.3 ± 52.1b
(<0.01)
(<0.013)
0.034
T3
ng/ml
2.80 ± 0.01
2.92 ± 0.14
1.16 ± 0.03
1.22 ± 0.04
<0.01
0.235
0.688
T4
ng/ml
98.6 ± 3.5
81.5 ± 5.2
41.6 ± 1.5
33.7 ± 0.9
<0.01
<0.01
0.172
TSH
μIU/ml
6.71 ± 0.47
7.06 ± 0.40
0.99 ± 0.23
1.85 ± 0.75
<0.01
0.236
0.617
Female
WT ()
db/db ()
WT ()
db/db ()
Age
Genotype
Interaction
Body weight
g
6.01 ± 0.11a
6.37 ± 0.14a
19.38 ± 0.21b
39.27 ± 0.92c
(<0.01)
(<0.01)
<0.01
Milk intake
g/h
0.093 ± 0.024
0.102 ± 0.031NS
Food intake
g/day
3.03 ± 0.09
7.34 ± 0.43∗∗
WT: wild type (homozygous wild type in leptin receptor gene); db/db: leptin receptor deficiency (homozygous mutants in leptin receptor gene); BW: body weight; WAT: white adipose tissue; BAT: brown adipose tissue; TG: triglyceride; T3: triiodothyronine; T4: thyroxine; TSH: thyroid-stimulating hormone. #The number of male samples for milk intake measurement was 7. All data were obtained from mice after 3 h fasting except milk and food intake (described in detail in Materials and Methods). Values are the mean ± SE. Values except milk and food intake were analyzed by two-way ANOVA (interaction, age, and genotype) followed by the Tukey-Kramer method when significant. Bold values mean the significance in the interaction. In the case, the main effect of age and genotype is shown by the parenthesis as a reference. Values of milk and food intake were analyzed by unpaired Student’s t-test, respectively. The same superscript letter and NS indicate that the difference was not significant. vs. WT mice at the respective age.