TAK-875 Mitigates β-Cell Lipotoxicity-Induced Metaflammation Damage through Inhibiting the TLR4-NF-κB Pathway

Figure 2

TLR4-NF-κB is involved in the protective effect of TAK-875 on lipotoxicity. (a, b) TLR4 protein expression was detected by western blotting in TLR4-overexpressing and siRNA-transfected β-cells. (c, d) Regulation of TLR4 expression altered the protective effect of TAK-875 on PA-induced β-cells apoptosis (c), and insulin secretion disorder (d). ^a vs. NC group, ^b vs. NC+vector group, ^c vs. 0.5 mmol/L PA group, ^d vs. 0.5 mmol/L PA+100 nmol/L TAK-875 group, and ^e vs. 0.5 mmol/L PA+100 nmol/L TAK-875+TLR4 siRNA group. (e, f) Activation of TLR4 activity decreased the effect of TAK-875 on inhibition of PA-induced apoptosis (e) and insulin secretion disorder (f). (g, h) Activation of NF-κB activity decreased the effect of TAK-875 on inhibited PA-induced apoptosis (g) and insulin secretion disorder (h). ^a vs. NC group and ^c vs. 0.5 mmol/L PA+100 nmol/L TAK-875 group.

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