Use of a Combined Technology of Ultrasonication, Three-Phase Partitioning, and Aqueous Enzymatic Oil Extraction for the Extraction of Oil from Spirogyra sp.

Figure 2

Effect of the use of papain on oil yield. The algal suspension was given a treatment of ultrasonication as described in the method section and was incubated at 40°C with crude papain (2 g) at pH 6 with magnetic stirring along with a control (without enzyme) and then a Three-Phase Partitioning was done as described before with 50% ammonium sulphate. In each case the yield of the oil was expressed in w/w, dry weight of the algae. Each set was performed in triplicate and the deviation between the experimental data was within 3%.