Use of a Combined Technology of Ultrasonication, Three-Phase Partitioning, and Aqueous Enzymatic Oil Extraction for the Extraction of Oil from Spirogyra sp.

Figure 3

Activation of papain by TPP. The effectiveness of papain (present during the course of TPP) was measured in terms of oil yield with a control. The control was an AEOE under similar conditions. The presonicated algal suspension was incubated with papain at pH 6 at 60°C for 6 h and then the oil was extracted in t-butanol saturated with ammonium sulphate in a biphasic medium. Each experimental set was run in triplicate and the variation in the data was within 3%.