Table 3: This table summarizes the bacteria and endotoxins identified in various bulk samples taken from the home (EMSL Method M009) and by RealTime Laboratories (RTL), Dallas, TX.
(a)

SampleSample #MediaTemp (°C)Analytical sensitivity CFU/gBacteriaColony countCFU/g

Plastic sheeting, crawl space#34SBA3598.000Bacillus sp
Streptomyces sp.
Actinomycetes
25 2.450.000

Moist gravel, crawl space#27SBA135885B. megaterium
Bacillus sp.
Total
10
7
17
8.850
6.190
15.000

Moist dirt, crawl space#28SBA1358130B. megaterium
Bacillus sp.
Total
4
6
10
32.500
48.800
81.300

Swab of wood, crawl space#25SBA23510.000Microbacterium hominis
Staphylococcus sp (not aureus)
Total
972
2
974
9.720.000
20.000
9.740.000

Dirt crawl space#28Blood Agar353Bacillus sp.
Proteus sp
Pseudomonas sp.
TNC4TNC4

Gravel, crawl space#27Blood Agar353Bacillus sp.
Proteus sp.
Pseudomonas sp.
TNC4TNC4

Sandal, under master bed#36Blood Agar353Bacillus sp.
Proteus sp.
Pseudomonas sp.
TNC4TNC4

(b)

EndotoxinsSample #Sample typeLocationConcentration (EU/Swab)5

#3SwabJ-Tube, Under Sink4930
#4SwabTop, Kitchen Cabinet24.800
BlankSwabField BlankNone Detected
BlankSwabLab BlankNone Detected

1These samples were tested to determine the major species of Bacillus.
2This sample was tested for Actinomycetes because of white mycelia type growth on wood truss.
3These samples were tested by RealTime Laboratories for the presence of bacteria species on samples tested for mycotoxins.
4CFU was not determined. TNTC: too numerous to count.
5Endotoxins were analyzed by ESML using LAL Kinetic Chromogenic Assay.