Cation-Specific Effects on the Self-Assembly of Collagen Molecules Mediated by Acetate on Mica Surface Observed with Atomic Force Microscopy

Table 1

Width, height, roughness (Ra, Rq), and surface area difference of the collagen molecular self-assembly in different collagen, KAc, NaAc, MgAc₂, and CaAc₂ concentrations.

Cation	Concentration	Width/nm	Height/nm	Ra/nm	Rq/nm	Surface area difference
—	5 μg/mL	16 ± 4		0.142	0.188	0.311%
	10 μg/mL	27 ± 9	1.165 ± 0.077	0.233	0.317	0.313%
	20 μg/mL	41 ± 15	1.462 ± 0.030	0.296	0.364	0.340%
	50 μg/mL	42 ± 9	1.923 ± 0.168	0.385	0.493	0.662%
K^+	25 mM	49 ± 7	1.906 ± 0.104	0.489	0.572	0.412%
	50 mM	60 ± 24	1.413 ± 0.098	0.369	0.442	0.163%
	100 mM	95 ± 19	2.279 ± 0.029	0.651	0.781	0.275%
	300 mM	73 ± 30	3.349 ± 0.352	0.999	1.210	0.388%
Na^+	50 mM	24 ± 4	1.302 ± 0.252	0.264	0.349	0.633%
	100 mM	27 ± 4	1.397 ± 0.449	0.289	0.360	0.388%
	200 mM	46 ± 14	2.817 ± 0.170	0.591	0.743	0.625%
	400 mM	45 ± 8	1.401 ± 0.084	0.378	0.471	0.179%
Mg^2+	50 mM	43 ± 10	2.378 ± 0.078	0.544	0.188	0.501%
	100 mM	76 ± 14	2.665 ± 0.058	0.643	0.317	0.594%
	300 mM	81 ± 5	2.435 ± 0.143	0.604	0.364	0.208%
	400 mM	59 ± 17	2.056 ± 0.048	0.441	0.493	0.634%
Ca^2+	100 mM	38 ± 4	0.892 ± 0.021	0.168	0.233	0.147%
	200 mM	47 ± 8	1.319 ± 0.058	0.453	0.536	0.238%
	300 mM	56 ± 13	1.378 ± 0.068	0.346	0.516	0.375%
	400 mM	58 ± 10	1.083 ± 0.111	0.222	0.311	0.205%