Combining a COI Mini-Barcode with Next-Generation Sequencing for Animal Origin Ingredients Identification in Processed Meat Product

Table 2

Detection of organisms in prescribed meat mixture.

Method	Cloning sequencing			NGS
Sample ID	B1	B2	B3	C1	C2	C3
Total no. of detected clones/tags	127	122	125	2,012,052	2,054,514	2,042,428
Pangasianodon hypophthalmus (S1)	√	√	√	√	√	√
Alectoris chukar (S2)	√	√	√	√	√	√
Bos taurus (S3)	√	√	√	√	√	√
Bubalus bubalis (S4)		√		√	√	√
Litopenaeus vannamei (S5)	√	√		√	√	√
Gallus gallus (S6)				√	√	√
Ctenopharyngodon idella (S7)	√		√	√	√	√
Ovis aries (S8)	√	√	√	√	√	√
Decapterus maruadsi (S9)	√			√	√	√
Sus scrofa (S10)	√			√	√	√
Branchiostegus argentatus (S11)				√	√	√
Pampus chinensis (S12)				√	√	√
Ovis canadensis	√		√	√	√	√
Anser canagica				√	√	√
Sarcophaga josephi				√	√	√
Sarcophaga antilope				√	√
Homo sapiens				√	√	√
Opisthoplatia orientalis					√	√
Blattella germanica					√
Hypophthalmichthys nobilis				√	√

The species were detected beyond expectation.