Statistical Optimization of Fermentation Process Parameters by Taguchi Orthogonal Array Design for Improved Bioethanol Production

Table 2

Matrix layout of the L₂₅ Taguchi orthogonal array design.

Run/expt. no.	Recombinant GH5 cellulase*	Recombinant GH43 hemicellulase*	S. cerevisiae*	C. shehatae*	pH	Temperature
1	0.25	1	1	1	5	30
2	0.5	1.5	2	0.25	4.3	30
3	1	2	0.5	1.5	3	30
4	1.5	0.25	1.5	0.5	6	30
5	2	0.5	0.25	2	5.5	30
6	0.25	0.25	0.25	0.25	3	26
7	0.5	0.5	1	1.5	6	26
8	1	1	2	0.5	5.5	26
9	1.5	1.5	0.5	2	5	26
10	2	2	1.5	1	4.3	26
11	2	0.25	2	1.5	5	28
12	1.5	2	1	0.25	5.5	28
13	1	1.5	0.25	1	6	28
14	0.5	1	1.5	2	3	28
15	0.25	0.5	0.5	0.5	4.3	28
16	0.25	1.5	1.5	1.5	5.5	33
17	0.5	2	0.25	0.5	5	33
18	1	0.25	1	2	4.3	33
19	1.5	0.5	2	1	3	33
20	2	1	0.5	0.25	6	33
21	0.25	2	2	2	6	35
22	0.5	0.25	0.5	1	5.5	35
23	1	0.5	1.5	0.25	5	35
24	1.5	1	0.25	1.5	4.3	35
25	2	1.5	1	0.5	3	35

∗The values of levels in (%, v v⁻¹).