Statistical Optimization of Fermentation Process Parameters by Taguchi Orthogonal Array Design for Improved Bioethanol Production

Table 3

Comparison of unoptimized and Taguchi optimized SSF combinations with mixed pretreated wild grass.

SSF combination	Substrate concentration (%, w v^−1) and mode of SSF	Reducing sugar* (g L^−1)	Ethanol yield (g of ethanol g of pretreated substrate^−1)	Ethanol titre* (g L^−1)
GH5 + GH43 + S. cerevisiae + C. shehatae (unoptimized)	1%; shake flask	1.70±0.09	0.228	1.50±0.06
GH5 + GH43 + S. cerevisiae + C. shehatae (Taguchi optimized)	1%; shake flask	2.31±0.05	0.304	2.0±0.04
GH5 + GH43 + S. cerevisiae + C. shehatae (Taguchi optimized)	1%; bioreactor	4.02±0.03	0.472	3.10±0.07

∗The values correspond to the maximum reducing sugar and maximum ethanol at a particular time; values are mean ± SE (𝑛=3).