Histochemical and Immunohistochemical Study of Peripolar Cells in Sheep

Table 1

Primary antibodies used and informative data.

Antibody	Supplier	Dilution	Antigen retrieval	Primary antibody—incubation time
α-Smooth muscle actin	Novocastra (αsm-1)	1 : 50	Microwave—5 minutes	3 h RT
NSE	Zymed (Lote 20771997)	1 : 50	Microwave—5 minutes	2 h RT
Chromogranin A	Dako	1 : 50	Microwave—5 minutes (2x)	2 h RT
S100 protein	Novocastra	1 : 200	Microwave—5 minutes	1 h RT
Desmin	Dako (D33)	1 : 100	Microwave—5 minutes	2 h RT
Cox-2	Transduction Laboratories,; Kentucky (Clone SP-21)	1 : 50	Microwave—5 minutes (4x)	ON at 4.°C
AE1/AE3	Dako (AE1/AE3)	1 : 50	Microwave—5 minutes	2 h RT
Wide Spectrum Cytokeratin	Dako	1 : 1000	Microwave—5 minutes (2x)	2 h RT

RT: room temperature; ON: overnight.