Chemical Separation of Fixed Tissue Using Thermolysin

Figure 2

Photomicrograph of chinchilla utricular macula showing calretinin antibody-labeled calyx afferents. The peptide bonds connecting the otolithic membrane to the underlying sensory epithelium have been digested through the use of thermolysin, allowing immunohistochemistry techniques to localize the mechanosensory hair cells. Scale bar = 100 μm.