Search for Conditions to Detect Epigenetic Marks and Nuclear Proteins in Immunostaining of the Testis and Cartilage
Figure 4
Ratios of antigen-positive and -negative cells under different conditions of fixation time and antigen retrieval for the detection of various nuclear antigens in the growth plate cartilage. The ratios of antigen-positive and -negative cells under each different condition are shown as percentage bar charts for fixation as a whole embryo (a) and fixation as an isolated humerus (b). Orange: the proportion of positive cells per DAPI-stained cells, and gray: the proportion of negative cells per DAPI-stained cells. Antibodies used in this study, fixation time, and presence or absence of antigen retrieval (“AR+” and “AR−”) are shown in the lower part of the figure.
