Evaluation and Validation of a Semiautomatic Image Analysis Method: Quantification of Nares Cell Proliferation of Rhinella arenarum (Anura) Detected with the Avidin-Biotin Complex/Peroxidase, DAB

Figure 1

Outline of the methodology of replacement of image analysis methods. The main steps of this process correspond to image acquisition and analysis by manual and semiautomatic methods, where black lines indicate the classic process (yellow line corresponds to the gold standard) and red ones the proposed new steps with computational models as an additional tool.