Research Article

Evaluation and Validation of a Semiautomatic Image Analysis Method: Quantification of Nares Cell Proliferation of Rhinella arenarum (Anura) Detected with the Avidin-Biotin Complex/Peroxidase, DAB

Figure 3

Phantom image representing the PCNA immunolabeling of the Rhinella arenarum olfactory epithelium. Regions and objects of interest were marked with (B) background; (N+) immunopositive nuclei; (N−) immunonegative nuclei; and (P) pigments.