Research Article

Evaluation and Validation of a Semiautomatic Image Analysis Method: Quantification of Nares Cell Proliferation of Rhinella arenarum (Anura) Detected with the Avidin-Biotin Complex/Peroxidase, DAB

Table 1

Main parameters drawing how they were found and named both in real immunohistochemistry images and their phantoms counterpart.

ROIsMean intensity level Associated noise
Real IH imageLabelsPhantom

BackgroundB RGBB
Immunopositive nucleiN+ RGBN+
Immunonegative nucleiN− RGBN−
PigmentsP RGBP