Evaluation and Validation of a Semiautomatic Image Analysis Method: Quantification of Nares Cell Proliferation of Rhinella arenarum (Anura) Detected with the Avidin-Biotin Complex/Peroxidase, DAB

Table 4

Mean ± SD of the number of counted N+ objects with both methods under different conditions. values of the statistic test made are also showed.

Parameter	Interest value	Tested values	Percentage of counted N+ objects	value	Semiautomatic	value
			Manual
	6	2	94.3 ± 3.0	0.0668	98.3 ± 1.5	0.8768
		6	98.3 ± 1.5		98.3 ± 3.0
		11	97.5 ± 2.5		98.3 ± 3.0
		18	99.3 ± 1.5		99.3 ± 1.5
		24	99.3 ± 0.8		97.5 ± 2.5
	13	7	97.5 ± 0.0	0.1871	93.3 ± 3.6	0.1646
		13	98.3 ± 1.5		98.3 ± 3.0
		26	95.8 ± 3.6		95.8 ± 0.8
		40	95.0 ± 4.3		98.3 ± 3.6
		55	93.3 ± 0.8		96.8 ± 0.8
	122, 86, 81	133, 96, 91	95.5 ± 0.0	0.4012	98.5 ± 2.6	0.9874
		112,76,71	96.7 ± 2.6		98.3 ± 2.6
		122, 86, 81	98.3 ± 1.4		98.3 ± 2.3
	155, 132, 130	136, 128, 134	98.3 ± 8.3	0.8617	96.7 ± 8.3	0.6745
		68, 64, 67	98.3 ± 1.4		98.3 ± 2.3
	9	4.65	95.3 ± 20.3	0.4945	98.3 ± 8.3	0.5607
		9	98.3 ± 1.4		98.3 ± 2.3
		18.60	95.3 ± 20.3		95.3 ± 20.3