Evaluation and Validation of a Semiautomatic Image Analysis Method: Quantification of Nares Cell Proliferation of Rhinella arenarum (Anura) Detected with the Avidin-Biotin Complex/Peroxidase, DAB

Table 6

Average number of nuclei immunopositive area values and half obtained from the quantification of images of the Rhinella arenarum immunolabeled nares both with the manual and with semiautomatic methods. Indexes success and value of p obtained from comparing the number of immunopositive nuclei obtained with manual and semiautomatic method.

	Manual		Semiautomatic			p values
	Number of N+	Area (µm^2)	Number of N+	Area (µm^2)
St. 42	30.8 ± 6.7	13.311 ± 5.159	28.0 ± 6.7	11.261 ± 6.444	102%	0.279
St. 46	21.8 ± 8.6	14.227 ± 4.966	20.5 ± 8.8	14.012 ± 7.646	94%	0.680