The Effect of 17β-Estradiol Administration on Cutaneous Wound Healing in 24-Week Ovariectomized Female Mice

Figure 3

Inflammatory cells and plasma TNF-α levels. (a) The number of neutrophils per mm². The number of neutrophils in the OVX + 17β-estradiol group was significantly smaller than that in the OVX group on day 3. (b) Neutrophils (arrows) stained with antineutrophil antibody were observed in wound tissue on days 3 and 7. Bar 20 μm. (c) The number of macrophages per mm². The number of macrophages in the OVX + 17β-estradiol group was significantly smaller than that in the OVX group on day 3. (d) Macrophages (arrows) stained with anti-Mac-3 antibody were observed in wound tissue on days 3 and 7. Bar 20 μm. (e) Systemic TNF-α levels, determined by ELISA on serum samples. There were no significant differences between the three groups on days 3 and 7. Values are expressed as mean ± SD, 𝑛=4-5 for each group, ANOVA, t-test, or Tukey-Kramer; ^∗𝑃<0.05, ^∗∗𝑃<0.01: OVX versus OVX + 17β-estradiol.

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