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Developmental Immunology
Volume 1 (1990), Issue 2, Pages 105-112
http://dx.doi.org/10.1155/1990/71584

Delay of Early B-Lymphocyte Development by Gamma 2b Immunoglobulin Transgene: Effect on Differentiation-Specific Molecules

1Howard Hughes Medical Institute, UCLA, Los Angeles, California 90024, USA
2School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 191104, USA
3Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA

Received 15 February 1990; Accepted 5 May 1990

Copyright © 1990 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mice transgenic for γ2b Ig heavy chain were examined for alterations in B-cell differentiation and endogenous Ig gene rearrangement and expression. Fresh bone marrow from these mice was markedly reduced in BP-1+ cells and there were small reductions in B220+ and sIg+ cells. A-MuLV (Abelson murine leukemia virus) transformants from these bone marrow cells showed little alteration in Ig gene rearrangement and expression when compared to controls, however. Isolation of the B-lymphoid compartment from these mice in vitro using LBMC (lymphoid bone marrow cultures) enabled more detailed characterization of the effects of the transgene. LBMC derived from γ2b transgenic mice had similar growth kinetics, but a 4-5-week delay in the expression of endogenous mu Ig in comparison to control cultures. Nucleic acids derived from these early cultures prior to endogenous mu Ig expression showed reduced Ig JH rearrangements, some sterile mu transcription, low levels of BP-1 expression, and virtually undetectable TdT (terminal deoxynucleotidyl transferase) expression. Thus, this γ2b transgene appears able to affect early B-lymphocyte development.