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Developmental Immunology
Volume 4 (1995), Issue 2, Pages 85-92
http://dx.doi.org/10.1155/1995/10359

Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone

1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et Unité 184 de I'INSERM, Institut de Chimie Biologique, 11 rue Humann, Strasbourg 67085, France
2MRC Clinical Sciences Center, Royal Postgraduate Medical School, Hammersmith Hospital, Ducane Road, London W12 ONN, United Kingdom

Received 24 August 1994; Accepted 18 November 1994

Copyright © 1995 Hindawi Publishing Corporation.

Abstract

We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. This clone has the phenotype B220+, BP-1+ , HSA +, CD43+ λ5+ , and CD25-, and has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface MHC class II molecules, although to a much lesser extent than the mature M12.4.1 B-cell lymphoma. G12 cells can process and present the native Hen Egg Lysozyme (HEL) to an MHC class II-restricted T-cell hybridoma. The efficiency of presentation is inferior to that obtained with M12.4.1 cells. This is the first report where a pro-B cell can serve as APC in an MHC class II-restricted presentation.