Comparative Study of Regulatory T Cell Function of Human CD25⁺CD4⁺ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood

Figure 3

Suppressive effect of CD4⁺CD25⁺ T cells on cytokine production by CD4⁺CD25⁻ responder cells. CD4⁺CD25⁺ T cells and CD4⁺CD25⁻ responder cells were prepared by a magnetic microbead system. Cells were stimulated with anti-CD3/CD28-mAb-coated beads in the presence of antigen-presenting cells. The supernatants were removed after 48 hours of incubation with either CD4⁺CD25⁺ T cells (black bars), CD4⁺CD25⁻ responder cells (white bars), and cocultured cells (gray bars). TGF-β level in supernatants was measured using ELISA, and levels of other cytokines were measured using a cytometric bead array kit. Data are mean ±SD of 3 independent experiments.

(a)

(b)

(c)