Research Article

Comparative Study of Regulatory T Cell Function of Human T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood

Figure 6

Restoration of suppressive activity in expanded CB CD25+CD4+ T cells. (a) Freshly isolated CD4+CD25+ cells were used as Treg in Figure 2(b), and expanded as described in Section 2. Freshly isolated CD25āˆ’CD4+ T cells were used as responder cells in both primary and secondary assays without expansion. Cell proliferation was assessed by 3H-thymidine uptake on day 4. Data are obtained by experiments with samples of 3 different donors. (b) Freshly isolated CD4+CD25+ cells or expanded CD4+CD25+ cells were stained with anti-CD3, CD25, and CD45RA, and analyzed using FACSCalibur. The cells were gated for CD3+CD25+ cells and the expression of CD45RA was compared between freshly isolated CD4+CD25+ cells (open histogram) and expanded CD4+CD25+ cells (closed histogram). Data are representative of 3 different donors. (c) Expression of FOXP3 protein was compared between freshly isolated cells and expanded cells by Western blot. Data are representative of 3 different donors.
305859.fig.006a
(a)
305859.fig.006b
(b)
305859.fig.006c
(c)