Research Article
Comparative Study of Regulatory T Cell Function of Human T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood
Table 1
Combinations of antibodies used in FACS analysis.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(a)All Abs were added to cells except PI,
and cells were stained for 20 minutes. PI were added just before FACS
analysis. (b)All Abs were added to cells except anti-FOXP3-Ab and/or anti-CD152-Ab, and cells were stained for 20 minutes. After cell fixation and permeabilization, anti-FOXP3-Ab and anti-CD152-Ab were added for intracellular staining. |