Human Brain Microvascular Endothelial Cells and Umbilical Vein Endothelial Cells Differentially Facilitate Leukocyte Recruitment and Utilize Chemokines for T Cell Migration

<table><i>Confluent THBMEC cultures possess intercellular
TJs while HUVECs do not.</i> Three different passages of THBMECs and HUVECs were cultured
to confluence. Endothelial cells were stained with rabbit anti-human ZO-1 and
occludin antibodies followed by FITC conjugated goat anti-rabbit IgG. Digital
pictures were taken following visualization with a Leica Aristoplan laser
scanning confocal microscope. THBMEC cultures exhibited continuous ZO-1 and
occludin expression at sites of intercellular contact. In contrast, HUVEC
cultures showed punctate ZO-1 staining and no occludin immunoreactivity. Scale
bar 10 μm.</table>

Journal of Immunology Research

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Figure 1

Figure 1: Human Brain Microvascular Endothelial Cells and Umbilical Vein Endothelial Cells Differentially Facilitate Leukocyte Recruitment and Utilize Chemokines for T Cell Migration 

Figure 1 | Human Brain Microvascular Endothelial Cells and Umbilical Vein Endothelial Cells Differentially Facilitate Leukocyte Recruitment and Utilize Chemokines for T Cell Migration 