Human Brain Microvascular Endothelial Cells and Umbilical Vein Endothelial Cells Differentially Facilitate Leukocyte Recruitment and Utilize Chemokines for T Cell Migration

Figure 2

Confluent THBMEC cultures have a higher TEER and lower solute permeability than HUVEC. THBMECs and HUVECs were cultured to confluence on 24-well collagen-coated Transwell^TM inserts. TEER (a) was measure using an EVOM voltohmmeter. THBMECs possess higher TEER values. Solute permeability (b) was assessed by adding 100 μl of 1 mg/mL fluoresceinated dextran-70 in Transwell^TM inserts. Fluorescent recovery in the lower chamber (well) was measured after 15 minutes as stated in Section 2. Triplicate wells were measured and inserts without cultured endothelial cells were used as controls (data not shown). THBMEC cultures demonstrate a lower solute permeability. ^*indicates 𝑃<.05 compared with HUVECs.