Serial Assessment of Immune Status by Circulating CD8+ Effector T Cell Frequencies for Posttransplant Infectious Complications
Figure 4
Changes in CD45 isoforms of CD8+ T cells, CD27−CD28− subsets, IFN- producing cells and perforin expression after LDLT in a 53-year-old, female recipient who underwent LDLT to treat HCV-related liver cirrhosis. In (a), flow cytometry, using peripheral blood nuclear cells (PBNCs) the
lymphocytes were stained with monoclonal antibodies to CD45RO and CCR7. The representative dot plots show double-staining for CD8+CCR7/CD45RO on gated lymphocytes (i), which identified 4 subsets of CD8+: naive (CD45RO−CCR7+), central memory (CD45RO+CCR7+), effector memory (CD45RO+CCR7−), and effector T cells (CD45RO−CCR7−). Other dot plots show double-staining for CD27/CD28 on gated CD8+ T cells (ii), CCR7/IFN- on gated CD8+CD45RA+ cells (iii), perforin/CCR7 on gated CD8+CD45RO− cells (iv). Cells in quadrants are presented as ratios (%). Right low (c), proportions of perforin and IFN- expression are expressed as ratios (%) of CD8+ T
cells. Pre, pre-LDLT; Tac, tacrolimus. (b) N, naive T cells; E, effector T cells; CM, central memory T cells; EM, effector memory T cells; and HAI, hepatic artery flow interruption. (c) IFN-, interferon-gamma; and CD27−CD28−,
CD27−CD28− subsets.
