About this Journal Submit a Manuscript Table of Contents
Clinical and Developmental Immunology
Volume 2010 (2010), Article ID 418695, 10 pages
Review Article

Effects of Parathyroid Hormone on Immune Function

1Department of Medicine, Staten Island University Hospital, 475 Seaview Avenue, Staten Island, NY 10305, USA
2NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA
3Division of Nephrology, Staten Island University Hospital, 475 Seaview Avenue, Staten Island, NY 10305, USA

Received 13 March 2010; Accepted 15 June 2010

Academic Editor: Stuart Berzins

Copyright © 2010 Abdallah Sassine Geara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Parathyroid hormone (PTH) function as immunologic mediator has become interesting with the recent usage of PTH analogue (teriparatide) in the management of osteoporosis. Since the early 1980s, PTH receptors were found on most immunologic cells (neutrophils, B and T cells). The in vitro evaluations for a possible role of PTH as immunomodulator have shown inconsistent results mainly due to methodological heterogeneity of these studies: it used different PTH formulations (rat, bovine, and human), at different dosages and different incubating periods. In some of these studies, the lymphocytes were collected from uremic patients or animals, which renders the interpretation of the results problematic due to the effect of uremic toxins. Parathyroidectomy has been found to reverse the immunologic defect in patients with high PTH levels. Nonetheless, the clinical significance of these findings is unclear. Further studies are needed to define if PTH does have immunomodulatory effects.

1. Introduction

Infection remains a major cause of morbidity and mortality in patients with end-stage renal disease (ESRD). Hospitalization rates for infections have risen since 1993, 19% for pneumonia, 24% for cellulitis, and 29% for sepsis/bacteremia. Death from sepsis is 50 times higher in hemodialysis patients than in the general population even after accounting for other comorbidities [1, 2]. Several factors make patients with ESRD susceptible to infections; one of the most difficult causes to treat is the development of an acquired immune dysfunction associated with chronic kidney disease (CKD) and dialysis therapy [35]. The first evidence for this dysfunction came from early observations which showed that patients attending dialysis units had endemic outbreaks of hepatitis B [6]. In addition, the clinical course for dialysis patients that acquired hepatitis B was worse; 60% of these patients became chronic carriers as compared to only 5% in the general population. Studies of vaccine efficacy corroborated these findings and helped to elucidate the pathogenic mechanism behind the immune defect. When patients with ESRD were vaccinated with protein-based vaccines, such as hepatitis B [7], influenza virus [8], and clostridium tetani [9], which require a T-lymphocyte-dependent response, there were high failure rates. In contrast, effective immunity was achieved using polysaccharide pneumococcal vaccine since this vaccine activates directly B cells without interaction with T-lymphocyte [10]. These observations and subsequent studies support the concept of an acquired T-lymphocyte dysfunction in ESRD patients. Though patients with ESRD are lymphopenic compared to healthy subjects, this effect is slight and would not explain the significant immune defect seen in this population [11].

In vitro studies examining T-lymphocyte function during exposure to various mitogens demonstrate a significant impairment in T-lymphocyte proliferation [12] and a reduction in activation-dependent cytokine production (interleukin-2,6,10, -interferon, and tumor necrosis factor alpha) [13]. This decrease in T-lymphocyte function was further identified to be an impairment in the interaction between antigen-presenting cells (APCs) and T-lymphocytes [3]. Therefore, although patients with ESRD have functionally normal T and B lymphocyte, the T and B lymphocytes do not respond appropriately, as they are not receiving normal signals from APCs. This impairment occurs via two mechanisms [3, 14]. Firstly, uremia causes a reduction of the essential costimulatory molecule B7-2 (CD86) on antigen-presenting cells which decreases the activation of helper T-lymphocyte cells [15]. This effect can be improved with dialysis therapy. Secondly, however, hemodialysis itself causes activation of antigen-presenting cells [14], which in addition to causing an immunity impairment, is now also believed to be responsible for the malnutrition-inflammation-atherosclerosis syndrome seen in patients with ESRD [16]. Similarly, a polymorphonuclear leukocyte (PMNL) dysfunction has been observed in dialysis patients with impairment in migration, chemotaxis, and reduced ability to kill intracellular micro-organisms [17].

Over the last 3 decades, secondary hyperparathyroidism in ESRD patients has been studied as a possible factor in the development of an acquired immune dysfunction [18]. PTH is an 84-amino acid polypeptide secreted by the parathyroid gland. Its homeostasis is mainly controlled by calcium/phosphorus regulation. High levels of PTH have been implicated in several complications associated with uremia. PTH adversely affects the metabolism of various cells and organs; it causes osteitis fibrosa cystica, cardiac arrhythmias [19, 20], peripheral neuropathy [21], anemia (by inhibiting precursors of erythropoiesis) [22, 23], and glucose intolerance [24, 25].

Kaplan et al. in the early 1970s [26] reported an increase in malignant neoplasms discovered at autopsy in patients with primary hyperparathyroidism. Similarly, there have been reports of an increase in the incidence of leukemia [27] and monoclonal gammopathy [28] in patients with primary hyperparathyroidism. Though these observations could not be definitively linked to excess PTH, the concept that PTH may modulate the immune response was further supported by Perry et al. [29] in describing a receptor for PTH in circulating human lymphocytes. While the immune defect in CKD appears to be multifactorial, the contribution of PTH, if any, remains unclear. This potential immune impairment of PTH is clinically relevant, since in theory a parathyroidectomy or medical treatment may reverse this effect. We present a narrative review of the literature relevant to PTH as an immune modulator and examine the effects of hyperparathyroidism on immune function in patients with CKD.

2. Parathyroid Hormone Receptors and Leukocytes

The observation that several immunodeficiency syndromes had associated bone abnormalities has led to the search for a link between leukocytes and bone cells (osteoblasts and osteoclasts). Various cytokines have osteoclasts as target sites [3034]. These cytokines affected bone remodeling by activating a PTH receptor on the monocyte-like precursor of osteoclasts [30]. Yamamoto et al. [35] were the first to identify the presence of PTH receptors on bovine lymphocytes. Perry et al. [29] demonstrated PTH receptors on human mononuclear cells and subsequently identified receptors on additional leukocytes [36]. The mechanism by which PTH influences leukocytes is not well understood, yet there appears to be an increase in intracellular calcium level. Potentially, this might lead to an increase in cellular adenylate cyclase activity [29, 35]

3. Parathyroid Hormone and T Lymphocytes (Table 1)

Studies examining the effect of PTH on T lymphocytes are neither consistent nor conclusive (see Table 1). Most studies showed that PTH produces an inhibitory effect on various parameters of the immune system [3739], while other studies demonstrated that PTH had a stimulatory function under certain laboratory conditions [40, 41].

Table 1: Study discussing the effect of PTH on T-lymphocytes.

Shasha et al. [37] were one of the first to show that when peripheral blood lymphocytes from healthy donors were incubated with increasing concentrations of PTH, a marked inhibition of E rosette formation was produced. Massry et al. [46] found a direct correlation between PTH levels in uremic patients and the degree of inhibition of lymphocyte proliferation. More recently, Kaneko et al. [39] found an decrease in T-lymphocyte proliferation from patients with ESRD when recombinant PTH (rPTH) was added in vitro and that the decrease in proliferation was dose-dependent. In the control group, which consisted of T lymphocytes isolated from healthy adults, proliferation was increased by addition of rPTH. This stimulatory effect on proliferation was reversed and inhibited by adding urea or an acid ( ). The authors concluded that the T-lymphocyte response to PTH is modulated by the uremic state.

In examining the other human studies that found a stimulatory effect of PTH on leukocytes obtained from healthy donor volunteers [37], the PTH used was bovine (not human), and the lymphocytes were incubated for longer periods (5 days). These differences may account for the inconsistent study results, making it difficult to determine if PTH affects the immune system in the clinical setting.

In addition, the results of in vitro assays are difficult to correlate to patients with CKD. Doses of hormones in laboratory studies use concentration ranges only as low as 2 to 1.6  M, while clinically, PTH is measured in pg/ml. Even in ESRD patients that develop severe tertiary hyperparathyroidism with PTH levels that may rise above 1000 pg/ml to levels in the upper range of 3000 pg/ml (about 315 picomoles) [46], serum PTH levels are still several fold lower than the concentration used in the in vitro studies.

Overall, results from laboratory assays alone are difficult to interpret, especially since they only provide some insight into the effects of acute exposure to PTH. Chronic exposure to PTH may affect immune cells differently; thus in vitro data needs to be evaluated with caution. Our laboratory recently completed a study evaluating the effects of PTH on the production of IL-6 and IL-8 from activated leukocytes from healthy donor volunteers (work still in press). Our results demonstrated no effect of PTH on the production of IL-6 and IL-8 and that leukocyte proliferation was inhibited only at the highest dose of PTH (0.8  M) tested.

Few studies exist that attempt to categorize changes of peripheral blood leukocytes in CKD patients. One described a slight lymphopenia compared to the leukocyte count in normal subjects [4749]. Another studied the effect of PTH on the distribution of T-lymphocytes subpopulations (CD4 lymphocytes, CD8 lymphocytes, and CD4/CD8 ratio) (see Table 2) [4245]. Ozdemir et al. [45] found that in ESRD patients the CD4/CD8 lymphocyte ratio was increased in the presence of high serum PTH levels. In contrast, Angelini et al. [42] studied patients with ESRD and found that patients with elevated PTH had a decrease in CD4, an increase in CD8 lymphocytes, and thus a decrease in the CD4/CD8 lymphocyte ratio. There was a linear correlation between the levels of PTH and CD8 lymphocytes and a reverse correlation between level of PTH and total T-lymphocytes, CD4 lymphocytes, and CD4/CD8 ratio. Klinger et al [40] found that (1–84) PTH stimulated proliferation of T lymphocytes in a dose-dependent manner, and that the hormone did not alter the CD4/CD8 ratio. Inactivation of PTH cancelled this stimulatory effect.

Table 2: Study of the effects of parathyroidectomy.

4. Restoration of T-Lymphocytes Function: Parathyroidectomy and Calcium Channel Blockers (Table 2)

Giacchino et al. [54] reported that the inhibitory capacity of serum taken from uremic patients on E rosette formation was decreased following parathyroidectomy. Shasha et al. [50] examined T-cell function in primary hyperparathyroidism both before and 1 month after parathyroidectomy. The hyperparathyroid patients, prior to surgery, demonstrated low total T-lymphocytes count, increased CD8 lymphocytes, decreased CD4/CD8 ratios, and a decreased ability of T-lymphocytes to become activated in comparison to healthy controls. All of these abnormalities were restored following parathyroidectomy. Similarly, Kotzmann et al. [53] reported a restoration of lymphocyte responsiveness to stimulation 6 months after parathyroidectomy in patients with primary hyperparathyroidism. Tzanno-Martins et al. [44] studied the consequence of parathyroidectomy in hemodialysis patients and showed that patients with extremely high levels of PTH had a complete restoration of impaired T-lymphocyte proliferation after parathyroidectomy.

5. Parathyroid Hormone and B Lymphocytes

Although the initial evidence found during the studies of vaccination in CKD was more suggestive of an indirect effect of PTH on B lymphocytes via T-lymphocytes dysfunction, the discovery of PTH receptors on B lymphocytes has favored a more direct effect of PTH. This has been found in several clinical experiments, where PTH was found to affect several aspects of the B-cell function (proliferation, antibodies production, and metabolism) (Table 3).

Table 3: Study discussing the effect of PTH on B-lymphocytes.

Alexiewicz et al. [38] found that both the intact molecule of (1–84) PTH and its amino-terminal fragment (1–34) PTH caused dose-dependent inhibition of B-lymphocyte proliferation in normal subjects. After the activity of the amino-terminal region was inhibited through oxidation of the SH residues, the inhibitory effect was preserved, suggesting that the mechanism of PTH is mostly mediated through the carboxyl-terminal region. This inhibitory influence is most likely mediated by the stimulation of cyclic AMP production. Furthermore, Gaciong et al. [51, 56] reported that the defect in antibody production in uremic patients was due to the direct action of PTH on B lymphocytes, and that B lymphocytes from ESRD patients produce very low amounts of IgG following T-cell stimulation in vitro.

Clinically, the plasma levels of IgG, IgM, and IgA are usually in the normal range in uremic patients, while specific antibody responses are significantly depressed [57, 58]. The response to vaccination against hepatitis B was used as a sensitive clinical marker for B-cell dysfunction [7]. Deficient reaction to vaccination was also documented for influenza [8], tetanus [9], and diphtheria. Pneumococcal vaccination is an exception, as uremic patients responded with normal antibody titers to each antigen type [10]. Since B lymphocytes recognition of polysaccharide antigens is a T-cell independent interaction, vaccination using polysaccharide antigens in the pneumococcal vaccine was sufficient. This implies that the defect in antibody production was more due to T-cell-B-cell interaction.

Since PTH function on B-lymphocyte cells was mediated through alteration of intracellular calcium metabolism, by using the calcium channel blocker nifedipine, Alexiewicz et al. [59, 60] were able to reverse the abnormalities of intracellular Ca concentration and restore adequate proliferation of B cells following stimulation.

6. PTH and Polymorphonuclear Leukocytes

PMNLs of patients with elevated PTH serum levels presented impaired migration [61], reduced phagocytic [62] and bactericidal activity [63], and an inhibited chemotaxis [64].

The first observations were found in patients with primary hyperparathyroidism and normal renal function. The migration and chemotaxis of PMNLs were impaired, however these abnormalities disappear after parathyroidectomy [64]. Tuma et al. [65] demonstrated that secondary hyperparathyroidism is either directly or indirectly responsible for the altered leukocyte function in patients with uremia, particularly those with marked elevation of PTH. Massry et al. [66] verified that (1–84) PTH stimulated elastase release from PMNL in a dose-dependent and time-dependent manner. This effect was mostly mediated by the carboxyl-terminal region of the hormone (1–34), and PTH had no stimulatory effect on elastase release. In addition, Doherty et al. [61] demonstrated that migration of PMNL from patients with advanced renal failure was reduced and that there is an inverse relationship between random migration of PMNLs and serum levels of PTH. Esposito et al. [67] studied the role of PTH in depressing PMNLs phagocytic function in uremia. The data produced shows that phagocytosis is lowered in uremic patients with both low and high plasma PTH but more noticeably in the group with high plasma PTH. Similarly, the contact angle of cells during phagocytosis was affected more in patients with high PTH levels.

The mechanism through which PTH impaired PMNL function is multifactorial. Alexiewicz et al. [62] found that chronic exposure to excess PTH may cause accumulation of calcium in PMNLs that leads to derangement of the intracellular cascade, which occurs during the process of phagocytosis (especially after the Fc gamma RIII receptors of PMNLs interact with antibody fixing the antigen). Horl et al. [68] found that in ESRD patients, PTH not only elevated basal levels of cytosolic calcium, it also altered carbohydrate metabolism. Glucose uptake, glycogen synthetase activity, and glycogen content were all reduced. Kiersztejn et al. [69] also found that both basal and stimulated O2 consumption of PMNLs in CKD subjects and rats was lower than normal.

Since increased intracellular calcium is the principal mechanism in PMNL dysfunction, calcium channel blocker therapy has been tested with some success. Studies in CKD rats demonstrate that these derangements in PMNLs could be prevented by a previous parathyroidectomy or treatment with verapamil. In ESRD patients, eight to nine weeks of verapamil therapy (120 mg/day) normalized the elevated intracellular calcium concentration and carbohydrate metabolism in PMNLs. However, these beneficial effects were lost after eight to ten weeks of discontinuation of verapamil treatment [68].

Alexiewicz et al. [60] found that treatment with nifedipine was associated with the return of intracellular calcium concentration toward normal values; it also restored the ATP content of PMNLs. The normalization of intracellular calcium concentration and restoration of PMNL dysfunction by long-term therapy with calcium channels blockers were also demonstrated in diabetic patients treated with amlodipine [70]. Five months after stopping nifedipine treatment, the positive activity of calcium channel blockers on phagocytosis was lost [61]. Haag-Weber et al. [71] found that continuous infusion of nifedipine in a dose of 18 micrograms/kg/h during HD completely inhibited the rise of cytosolic free calcium during dialysis. PMNL dysfunction could be reversed by parathyroidectomy [65, 69].

7. Explanation of the Discrepancy between In Vitro and In Vivo Studies

Although the current published studies demonstrate that PTH can influence various parameters of the immune system in both normal and ESRD subjects, the results of the laboratory studies are inconsistent, and their clinical significance still needs further investigation. What prevents a more definitive conclusion to be made when examining and comparing these in vitro studies is that the dosages of PTH used in these laboratory studies are much higher than the physiological ranges of PTH. Even compared to the abnormally high ranges seen in ESRD patients with tertiary hyperparathyroidism, the doses used in laboratory experiments were several logs higher. However, the effect of this higher concentration is decreased by the fact that leukocytes counts in wells during in vitro studies are also several log higher than blood leukocytes in patients. Therefore, the ratio of PTH to leukocytes may be more important to reflect in vivo conditions. Furthermore, chronic exposure that occurs in CKD is difficult to study in vitro, since human leukocytes cultures are short term ( 5 days). A possible model for studying chronic exposure of PTH in laboratory experiments would be to use cell lines from immortalized human leukocytes. However, no good laboratory model has been developed for examining chronic exposure to PTH in leukocytes to date. Another difficulty in experimental studies was that various sources of PTH were used (rat, bovine, and human) and varying conditions of culture. Although there are limitations and difficulties in interpreting these studies, it remains that PTH receptors are located on leukocytes, and their exact role and function need to be defined. One possible way to determine if hyperparathyroidism associated with ESRD affects immune function is to examine if PTH receptors have a physiologic function in health. This approach seems more reasonable than starting with ESRD patients who already have various metabolic alterations.

8. Conclusion

Many of the infectious complications experienced by patients undergoing hemodialysis can be attributed to altered host defenses. Both humoral and cellular-mediated immunities are affected in ESRD patients. Alterations of the immune system in ESRD patients are multifactorial. The evidence presented shows that PTH may be an important factor in influencing this dysfunction. Massry and Fadda. [72] stated in his review that chronic renal failure is a “state of cellular calcium toxicity.” PTH is an essential hormone that initiates the cascade of events leading to increased calcium influx intracellularly, a pivotal step in modulating leukocyte enzymes and biochemical processes.

In conclusion, we present a review of the literature evaluating PTH and immune function. After observing that PTH receptors are located on various immune cells and evaluating the studies that attempt to understand the role of PTH in disease, it is clear that a basic understanding of the normal physiology of the PTH receptor on leukocytes is imperative. Finally, evidence of PTH affecting immunity may not be solely achieved by laboratory studies. Epidemiologic and observational studies should be done from large, maintained databases of ESRD patients, such as the US Renal Data System. Examination of infectious disease death rates and cancer rates can be correlated to PTH level. While the data from the reviewed studies supports the possibility that PTH affects the immune system, further research is needed, particularly since this abnormality could be reversed with the treatment of hyperparathyroidism.


The authors would like to thank Ms. Stephanie Bogin for her review and editing of the paper.


  1. M. J. Sarnak and B. L. Jaber, “Mortality caused by sepsis in patients with end-stage renal disease compared with the general population,” Kidney International, vol. 58, no. 4, pp. 1758–1764, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. P. C. W. van Dijk, K. J. Jager, F. de Charro et al., “Renal replacement therapy in Europe: the results of a collaborative effort by the ERA-EDTA registry and six national or regional registries,” Nephrology Dialysis Transplantation, vol. 16, no. 6, pp. 1120–1129, 2001. View at Scopus
  3. M. Girndt, M. Sester, U. Sester, H. Kaul, and H. Köhler, “Molecular aspects of T- and B-cell function in uremia,” Kidney International, vol. 59, no. 78, pp. S206–S211, 2001. View at Scopus
  4. C. J. Kelly, “T cell function in chronic renal failure and dialysis,” Blood Purification, vol. 12, no. 1, pp. 36–41, 1994. View at Scopus
  5. E. L. Pesanti, “Immunologic defects and vaccination in patients with chronic renal failure,” Infectious Disease Clinics of North America, vol. 15, no. 3, pp. 813–832, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. W. T. London, M. Di Figlia, A. Sutnick, and B. S. Blumberg, “An epidemic of hepatitis in a chronic-hemodialysis unit. Australia antigen and differences in host response,” New England Journal of Medicine, vol. 281, no. 11, pp. 571–578, 1969. View at Scopus
  7. H. Kohler, W. Arnold, G. Renschin, H. H. Dormeyer, and K. H. Meyer zum Büschenfelde, “Active hepatitis B vaccination of dialysis patients and medical staff,” Kidney International, vol. 25, no. 1, pp. 124–128, 1984.
  8. P. Rautenberg, I. Teifke, T. Schlegelberger, and U. Ullmann, “Influenza subtype-specific IgA, IgM and IgG responses in patients on hemodialysis after influenza vaccination,” Infection, vol. 16, no. 6, pp. 323–328, 1988. View at Scopus
  9. M. Girndt, M. Pietsch, and H. Kohler, “Tetanus immunization and its association to hepatitis B vaccination in patients with chronic renal failure,” American Journal of Kidney Diseases, vol. 26, no. 3, pp. 454–460, 1995. View at Scopus
  10. E. A. Friedman, M. M. Beyer, S. R. Hirsch, and G. Schiffman, “Intact antibody response to pneumococcal capsular polysaccharides in uremia and diabetes,” Journal of the American Medical Association, vol. 244, no. 20, pp. 2310–2311, 1980. View at Publisher · View at Google Scholar · View at Scopus
  11. L. Chatenoud, B. Dugas, G. Beaurain, et al., “Presence of preactivated T cells in hemodialyzed patients: their possible role in altered immunity,” Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 19, pp. 7457–7461, 1986. View at Scopus
  12. S. C. Meuer, M. Hauer, P. Kurz, K.-H. Meyer zum Buschenfelde, and H. Kohler, “Selective blockade of the antigen-receptor-mediated pathway of T cell activation in patients with impaired primary immune responses,” Journal of Clinical Investigation, vol. 80, no. 3, pp. 743–749, 1987. View at Scopus
  13. M. Girndt, H. Kohler, E. Schiedhelm-Weick, J. F. Schlaak, K.-H. Meyer Zum Buschenfelde, and B. Fleischer, “Production of interleukin-6, tumor necrosis factor α and interleukin-10 in vitro correlates with the clinical immune defect in chronic hemodialysis patients,” Kidney International, vol. 47, no. 2, pp. 559–565, 1995. View at Scopus
  14. T. Eleftheriadis, G. Antoniadi, V. Liakopoulos, C. Kartsios, and I. Stefanidis, “Disturbances of acquired immunity in hemodialysis patients,” Seminars in Dialysis, vol. 20, no. 5, pp. 440–451, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  15. M. Girndt, M. Sester, U. Sester, H. Kaul, and H. Köhler, “Defective expression of B7-2 (CD86) on monocytes of dialysis patients correlates to the uremia-associated immune defect,” Kidney International, vol. 59, no. 4, pp. 1382–1389, 2001. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  16. P. Stenvinkel, O. Heimbürger, B. Lindholm, G. A. Kaysen, and J. Bergström, “Are there two types of malnutrition in chronic renal failure? Evidence for relationships between malnutrition, inflammation and atherosclerosis (MIA syndrome),” Nephrology Dialysis Transplantation, vol. 15, no. 7, pp. 953–960, 2000. View at Scopus
  17. M. Chonchol, “Neutrophil dysfunction and infection risk in end-stage renal disease,” Seminars in Dialysis, vol. 19, no. 4, pp. 291–296, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  18. R. Shurtz-Swirski, T. Shkolnik, and S. M. Shasha, “Parathyroid hormone and the cellular immune system,” Nephron, vol. 70, no. 1, pp. 21–24, 1995. View at Scopus
  19. D. M. Voss and E. H. Drake, “Cardiac manifestations of hyperparathyroidism, with presentation of a previously unreported arrhythmia,” American Heart Journal, vol. 73, no. 2, pp. 235–239, 1967. View at Scopus
  20. E. Bogin, S. G. Massry, and I. Harary, “Effect of parathyroid hormone on rat heart cells,” Journal of Clinical Investigation, vol. 67, no. 4, pp. 1215–1227, 1981. View at Scopus
  21. G. N. Rizzo and C. W. Olanow, “Neurologic manifestations of hyperparathyroidism. Study of 120 cases,” Arquivos de Neuro-Psiquiatria, vol. 40, no. 3, pp. 213–222, 1982. View at Scopus
  22. D. Myetes, E. Bogin, A. Ma, P. P. Dukes, and S. G. Massry, “Effect of parathyroid hormone on erythropoiesis,” Journal of Clinical Investigation, vol. 67, no. 5, pp. 1263–1269, 1981. View at Scopus
  23. E. Bogin, S. G. Massry, J. Levi, M. Djaldeti, G. Bristol, and J. Smith, “Effect of parathyroid hormone on osmotic fragility of human erythrocytes,” Journal of Clinical Investigation, vol. 69, no. 4, pp. 1017–1025, 1982. View at Scopus
  24. S. Kumar, A. O. Olukoga, C. Gordoni et al., “Impaired glucose tolerance and insulin insensitivity in primary hyperparathyroidism,” Clinical Endocrinology, vol. 40, no. 1, pp. 47–53, 1994. View at Scopus
  25. S. Ayturk, A. Gursoy, N. B. Tutuncu, D. T. Ertugrul, and N. G. Demirag, “Changes in insulin sensitivity and glucose and bone metabolism over time in patients with asymptomatic primary hyperparathyroidism,” Journal of Clinical Endocrinology and Metabolism, vol. 91, no. 11, pp. 4260–4263, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  26. L. Kaplan, A. D. Katz, C. Ben-Isaac, and S. G. Massry, “Malignant neoplasms and parathyroid adenoma,” Cancer, vol. 28, no. 2, pp. 401–407, 1971. View at Scopus
  27. J. C. Wang, W. Steier, M. K. Aung, and M. S. Tobin, “Primary hyperparathyroidism and chronic lymphocytic leukemia,” Cancer, vol. 42, no. 4, pp. 1964–1969, 1978. View at Scopus
  28. R. N. Dexter, F. Mullinax, H. L. Estep, and R. C. Williams Jr., “Monoclonal IgG gammopathy and hyperparathyroidism,” Annals of Internal Medicine, vol. 77, no. 5, pp. 759–764, 1972. View at Scopus
  29. H. M. Perry III, J. C. Chappel, E. Bellorin-Font, J. Tamao, K. J. Martin, and S. L. Teitelbaum, “Parathyroid hormone receptors in circulating human mononuclear leukocytes,” Journal of Biological Chemistry, vol. 259, no. 9, pp. 5531–5535, 1984. View at Scopus
  30. H. E. Gruber, “Bone and the immune system,” Proceedings of the Society for Experimental Biology and Medicine, vol. 197, no. 3, pp. 219–225, 1991. View at Scopus
  31. P. Chen, C. Trummel, J. Horton, J. J. Baker, and J. J. Oppenheim, “Production of osteoclast activating factor by normal human peripheral blood rosetting and nonrosetting lymphocytes,” European Journal of Immunology, vol. 6, no. 10, pp. 732–736, 1976. View at Scopus
  32. G. Milhaud and M. L. Labat, “Thymus and osteopetrosis,” Clinical Orthopaedics and Related Research, vol. 135, pp. 260–271, 1978. View at Scopus
  33. T. Yoneda and G. R. Mundy, “Monocytes regulate osteoclast-activating factor production by releasing prostaglandins,” Journal of Experimental Medicine, vol. 150, no. 2, pp. 338–350, 1979. View at Scopus
  34. C. Minkin, L. Blackman, J. Newbrey, S. Pokress, R. Posek, and M. Walling, “Effects of parathyroid hormone and calcitonin on adenylate cyclase in murine mononuclear phagocytes,” Biochemical and Biophysical Research Communications, vol. 76, no. 3, pp. 875–881, 1977. View at Scopus
  35. I. Yamamoto, J. T. Potts Jr., and G. V. Segre, “Circulating bovine lymphocytes contain receptors for parathyroid hormone,” Journal of Clinical Investigation, vol. 71, no. 2, pp. 404–407, 1983. View at Scopus
  36. A. A. Bialasiewicz, H. Jueppner, V. Diehl, and R. D. Hesch, “Binding of bovine parathyroid hormone to surface receptors of cultured B-lymphocytes,” Biochimica et Biophysica Acta, vol. 584, no. 3, pp. 467–478, 1979. View at Scopus
  37. S. M. Shasha, B. Kristal, M. Barzilai, U. E. Makov, and T. Shkolnik, “In vitro effect of PTH on normal T cell functions,” Nephron, vol. 50, no. 3, pp. 212–216, 1988. View at Scopus
  38. J. M. Alexiewicz, M. Klinger, T. O. Pitts, Z. Gaciong, M. Linker-Israeli, and S. G. Massry, “Parathyroid hormone inhibits B cell proliferation: implications in chronic renal failure,” Journal of the American Society of Nephrology, vol. 1, no. 3, pp. 236–244, 1990. View at Scopus
  39. T. Kaneko, E. Osono, N. Hayama, Y. Lino, and A. Terashi, “T-cell activation modified by parathyroid hormone (PTH) in patients with end-stage renal disease,” Clinical Nephrology, vol. 48, no. 6, pp. 353–358, 1997. View at Scopus
  40. M. Klinger, J. M. Alexiewicz, M. Linker-Israeli et al., “Effect of parathyroid hormone on human T cell activation,” Kidney International, vol. 37, no. 6, pp. 1543–1551, 1990. View at Scopus
  41. E. Lewin, J. Ladefoged, L. Brandi, and K. Olgaard, “Parathyroid hormone dependent T cell proliferation in uremic rats,” Kidney International, vol. 44, no. 2, pp. 379–384, 1993. View at Scopus
  42. D. Angelini, A. Carlini, R. Giusti et al., “Parathyroid hormone and T-cellular immunity in uremic patients in replacement dialytic therapy,” Artificial Organs, vol. 17, no. 2, pp. 73–75, 1993.
  43. D. Angelini, A. Carlini, L. Mazzotta et al., “Immunologic disturbances and levels of parathyroid hormone in uremic patients in replacement dialysis therapy,” Clinical Nephrology, vol. 42, no. 4, pp. 246–250, 1994.
  44. C. Tzanno-Martins, E. Futata, V. Jorgetti, and A. J. S. Duarte, “Restoration of impaired T-cell proliferation after parathyroidectomy in hemodialysis patients,” Nephron, vol. 84, no. 3, pp. 224–227, 2000.
  45. F. N. Ozdemir, U. Yakupoglu, M. Turan et al., “Role of parathormone levels on T-cell response in hemodialysis patients,” Transplantation Proceedings, vol. 34, no. 6, pp. 2044–2045, 2002. View at Publisher · View at Google Scholar
  46. S. G. Massry, J. W. Coburn, G. M. Chertow et al., “K/DOQI clinical practice guidelines for bone metabolism and disease in chronic kidney disease,” American Journal of Kidney Diseases, vol. 42, no. 4, pp. S1–S201, 2003.
  47. J.-W. Yoon, S. Gollapudi, M. V. Pahl, and N. D. Vaziri, “Naïve and central memory T-cell lymphopenia in end-stage renal disease,” Kidney International, vol. 70, no. 2, pp. 371–376, 2006. View at Publisher · View at Google Scholar · View at PubMed
  48. M. Haag-Weber and W. H. Horl, “The immune system in uremia and during its treatment,” New Horizons, vol. 3, no. 4, pp. 669–679, 1995.
  49. J. L. Touraine, F. Touraine, J. P. Revillard, J. Brochier, and J. Traeger, “T lymphocytes and serum inhibitors of cell mediated immunity in renal insufficiency,” Nephron, vol. 14, no. 2, pp. 195–208, 1975.
  50. S. M. Shasha, B. Kristal, O. Steinberg, and T. Shkolnik, “Effect of parathyroidectomy on T cell functions in patients with primary hyperparathyroidism,” American Journal of Nephrology, vol. 9, no. 1, pp. 25–29, 1989.
  51. Z. Gaciong, J. M. Alexiewicz, and S. G. Massry, “Impaired in vivo antibody production in CRF rats: role of secondary hyperparathyroidism,” Kidney International, vol. 40, no. 5, pp. 862–867, 1991.
  52. I. Chervu, M. Kiersztejn, J. M. Alexiewicz, G. Z. Fadda, M. Smogorzewski, and S. G. Massry, “Impaired phagocytosis in chronic renal failure is mediated by secondary hyperparathyroidism,” Kidney International, vol. 41, no. 6, pp. 1501–1505, 1992.
  53. H. Kotzmann, M. Köller, C. Abela et al., “Effects of parathyroid hormone and serum calcium on the phenotype and function of mononuclear cells in patients with primary hyperparathyroidism,” European Journal of Clinical Investigation, vol. 28, no. 5, pp. 353–358, 1998. View at Publisher · View at Google Scholar
  54. F. Giacchino, G. Quattrocchio, and G. Piccoli, “Parathyroid hormone as a uremic toxin: its influence on T lymphocyte receptors,” Clinical Nephrology, vol. 23, no. 2, pp. 105–106, 1985.
  55. Y. Jiang, A. Yoshida, C. Ishioka, H. Kimata, and H. Mikawa, “Parathyroid hormone inhibits immunoglobulin production without affecting cell growth in human B cells,” Clinical Immunology and Immunopathology, vol. 65, no. 3, pp. 286–293, 1992. View at Publisher · View at Google Scholar
  56. Z. Gaciong, J. M. Alexiewicz, M. Linker-Israeli, I. A. Shulman, T. O. Pitts, and S. G. Massry, “Inhibition of immunoglobulin production by parathyroid hormone. Implications in chronic renal failure,” Kidney International, vol. 40, no. 1, pp. 96–106, 1991.
  57. A. B. Hauser, A. E. M. Stinghen, S. Kato et al., “Characteristics and causes of immune dysfunction related to uremia and dialysis,” Peritoneal Dialysis International, vol. 28, no. 3, pp. S183–S187, 2008.
  58. G. Cohen, M. Haag-Weber, and W. H. Horl, “Immune dysfunction in uremia,” Kidney International, vol. 51, no. 62, pp. S79–S82, 1997.
  59. J. M. Alexiewicz, M. Smogorzewski, M. Klin, M. Akmal, and S. G. Massry, “Effect of treatment of hemodialysis patients with nifedipine on metabolism and function of polymorphonuclear leukocytes,” American Journal of Kidney Diseases, vol. 25, no. 3, pp. 440–444, 1995.
  60. J. M. Alexiewicz, M. Smogorzewski, S. K. Gill, M. Akmal, and S. G. Massry, “Time course of the effect of nifedipine therapy and its discontinuation on [Ca2+](i) and phagocytosis of polymorphonuclear leukocytes from hemodialysis patients,” American Journal of Nephrology, vol. 17, no. 1, pp. 12–16, 1997.
  61. C. C. Doherty, P. LaBelle, J. F. Collins, N. Brautbar, and S. G. Massry, “Effect of parathyroid hormone in random migration of human polymorphonuclear leukocytes,” American Journal of Nephrology, vol. 8, no. 3, pp. 212–219, 1988.
  62. J. M. Alexiewicz, M. Smogorzewski, G. Z. Fadda, and S. G. Massry, “Impaired phagocytosis in dialysis patients: studies on mechanisms,” American Journal of Nephrology, vol. 11, no. 2, pp. 102–111, 1991.
  63. D. J. Salant, A. M. Glover, R. Anderson et al., “Polymorphonuclear leucocyte function in chronic renal failure and after renal transplantation,” Proceedings of the European Dialysis and Transplant Association, vol. 12, pp. 370–379, 1976.
  64. F. Khan, A. J. Khan, C. Papagaroufalis, J. Warman, P. Khan, and H. E. Evans, “Reversible defect of neutrophil chemotaxis and random migration in primary hyperparathyroidism,” Journal of Clinical Endocrinology and Metabolism, vol. 48, no. 4, pp. 582–584, 1979.
  65. S. N. Tuma, R. R. Martin, L. E. Mallette, and G. Eknoyan, “Augmented polymorphonuclear chemiluminescence in patients with secondary hyperparathyroidism,” Journal of Laboratory and Clinical Medicine, vol. 97, no. 2, pp. 291–298, 1981.
  66. S. G. Massry, R. M. Schaeffer, M. Teschner, M. Roeder, J. F. Zull, and A. Heidland, “Effect of parathyroid hormone on elastase release from human polymorphonuclear leucocytes,” Kidney International, vol. 36, no. 5, pp. 883–890, 1989.
  67. R. Esposito, C. Romano-Carratelli, N. Lanzetti et al., “Toxicity in uremia 2. Correlation between PTH levels and impaired aspecific immunity,” International Journal of Artificial Organs, vol. 11, no. 3, pp. 159–160, 1988.
  68. W. H. Horl, M. Haag-Weber, B. Mai, and S. G. Massry, “Verapamil reverses abnormal [Ca2+]i and carbohydrate metabolism of PMNL of dialysis patients,” Kidney International, vol. 47, no. 6, pp. 1741–1745, 1995.
  69. M. Kiersztejn, M. Smogorzewski, P. Thanakitcharu, G. Z. Fadda, and S. G. Massry, “Decreased O2 consumption by PMNL from humans and rats with CRF: role of secondary hyperparathyroidism,” Kidney International, vol. 42, no. 3, pp. 602–609, 1992.
  70. N. Seyrek, W. Marcinkowski, M. Smogorzewski, T. M. Demerdash, and S. G. Massry, “Amlodipine prevents and reverses the elevation in [Ca2+](i) and the impaired phagocytosis of PMNL of diabetic rats,” Nephrology Dialysis Transplantation, vol. 12, no. 2, pp. 265–272, 1997. View at Publisher · View at Google Scholar
  71. M. Haag-Weber, B. Mai, and W. H. Horl, “Effect of hemodialysis on intracellular calcium in human polymorphonuclear neutrophils,” Mineral and Electrolyte Metabolism, vol. 18, no. 2–5, pp. 151–155, 1992.
  72. S. G. Massry and G. Z. Fadda, “Chronic renal failure is a state of cellular calcium toxicity,” American Journal of Kidney Diseases, vol. 21, no. 1, pp. 81–86, 1993.