Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4
Figure 1
Binding affinity of peptides for HLA-A*0201 molecule. T2 cells were incubated with indicated concentrations of the peptides in serum-free RPMI 1640 medium supplied with 3 μg/mL human β2m at 37°C for 16 h. And then the cells were stained with anti-HLA-A2 mAb and FITC-labeled goat antimouse IgG. The expression of HLA-A*0201 on T2 cells was determined with FACS Calibur flow cytometer. The peptides HBcAg18–27 and OVA257–264 were taken as positive control and negative control, respectively. Each sample was measured in three replicates and the experiment was repeated three times.
