Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4
Figure 3
In vivo induction of peptide-specific CTLs. The HLA-A*0201/Kb transgenic mice were immunized with the peptides P1 and P3 prepared in IFA, respectively. Another group of mice were immunized with IFA without peptide as negative control. Mice were sacrificed 10 days after immunization and splenocytes were stimulated in vitro for 5 days with 2 μg/mL peptide and 10 units/mL rmIL-2 to expand them as effector cells. A cytotoxicity assay was used to evaluate the lysis of LB1751-MEL cells by peptide-stimulated splenocytes from corresponding peptide-immunized mice (P1 and P3), P1- or P3-stimulated splenocytes from IFA-immunized mice (IFA+P1 and IFA+P3), and the lysis of LB1751-MEL cells, which surface HLA-A*0201 molecules were blocked with ant-HLA-A*0201 mAb, by peptide-immunized mice (P1+HLA-A2 mAb and P3+ HLA-A2 mAb). The experiment was repeated three times.
