Potential Target Antigens for a Universal Vaccine in Epithelial Ovarian Cancer

Table 1

Antibodies used for immunohistochemical staining.

Antigen	Antigen retrieval	Clone	Dilution	Company
p53	Tris/EDTA (pH8)	DO-7^1	1 : 2000	DAKO^2
SP17	Citrate (pH 6)	Sp17MF1	1 : 100	3
survivin	Citrate (pH 6)	71G4B7E	1 : 100	Cell signaling^4
WT1	Tris/HCL (pH 9)	6F-H2	1 : 25	DAKO^2
NY-ESO-1	EDTA (pH 8)	E978	1 : 50	Zymed^5
HLA-A	Citrate (pH 6)	HCA2	1 : 500	6
HLA-B/C	Citrate (pH 6)	HC-10	1 : 100	6
𝛽2-m	Citrate (pH 6)	Polyclonal	1 : 400	DAKO^2

1Detects both wild-type and mutant p53 protein; 2DAKO, Glostrup, Denmark; 3The SP17 antibody kindly provided by Dr. Maurizio Chiriva, Texas Tech University; 4Cell Signaling, Danvers, USA; 5Zymed, San Francisco, USA; 6The HCA2 and HC-10 antibodies were a gift from Professor Dr. Neefjes, Netherlands Cancer Institute, Amsterdam, The Netherlands.