The Desmoplastic Stroma Plays an Essential Role in the Accumulation and Modulation of Infiltrated Immune Cells in Pancreatic Adenocarcinoma

Figure 5

PDAC tissues show infiltration of immune cells such as macrophages, dendritic cells, and CTLs. PDAC and healthy pancreatic tissue samples were immunohistochemically stained with anti CD163, S100, CD83, and CD8 antibodies (abs) followed by biotin-conjugated secondary abs and streptavidin-HRP complex. Peroxidase was detected using DAB chromogen. The amount CD163 (a), S100 (b), CD83 (c), and CD8 (d) positive cells/μm² were calculated from 20 randomly selected fields using a computerized image processing analysis system linked to a microscope. (e) The micrographs show expression and location of CD163, S100, CD83, and CD8 positive cells in the PDAC and healthy pancreatic tissues. Size bar 20 μm. *𝑃<0.05, **𝑃<0.05, ***𝑃<0.001.