Dendritic Cell-Based Vaccines Positively Impact Natural Killer and Regulatory T Cells in Hepatocellular Carcinoma Patients
Figure 2
FOXP3 expression in CD4+CD3+CD25hi (Treg) cells. Phenotyping of Treg cells from patients who received the pep/DC vaccine, showing longitudinal changes. “Pre” denotes PBMC from time point 0. “Post” denotes PBMC from postvaccine administration at time points available. Patient A1 tested at days 35 and 56; pt B2 at d28, 56, and 112; pt B5 at d14 and 28; pt B8 and d14 and 112, pt A4 at d14. (a) FOXP3 assessed intracellularly in CD3+CD4+CD25hi cells, showing MFI, or (b) % positivity. One-tail t-test values are: FOXP3 MFI: pre to post 1: 0.09, pre to post 2: 0.07, post 1 to post 2: 0.17; all other values higher and n.s. FOXP3 percent positive: pre to post 1: 0.03; pre to post 2: 0.10; all other values higher and n.s.
