IFN Regulatory Factors 4 and 8 Expression in the NOD Mouse
Figure 1
Age-related IRF4 and IRF8 mRNA expression levels in splenocytes of diabetes-prone and diabetes-resistant mice. Total splenic RNA was obtained from NOD, NOR, C57BL/6J, CD-1, and BALB/c mice, purified and gene expression profile of IRF4 (a) and IRF8 (b) were analyzed by real time RT-PCR. Each RNA sample was analyzed in triplicates and normalized to HPRT expression (ΔCt). Each NOD sample was normalized to a BALB/c sample (ΔΔCt = ΔCt sample − ΔCt BALB/c) and, the expression level was calculated as follows: 2−ΔΔCt. Data are shown as the average ± SD of 4 independent experiments (*, ** and ***).
